The Palatine Gallery (Italian: Galleria Palatina) is an art gallery in the Palazzo Pitti, Florence, Italy. It was opened to the public in 1769 by Grand Duke Pietro Leopoldo of Tuscany. The gallery has housed some of the most important works of art from the Medici collections.

Some of the notable paintings in the collection include works by Raphael, Titian, Caravaggio, and Rubens